MUMBAI, India, June 30 -- Intellectual Property India has published a patent application (202641075040 A) filed by Dr. B. Poornima; and Vijaya Institute Of Pharmaceutical Sciences Women on June 17, 2026, for Quantitative Estimation Of Ascorbic Acid And Correlation With Dpph Radical Scavenging Activity In Common Dietary Vegetables.
Inventors include Dr. B. Poornima; V. Madhu Sri; V. Nithya Sri; A. Harika Devi; I. Praseeda; and M. Kamili Jyothi.
The application for the patent was published on June 26, 2026, under issue no. 26/2026.
Abstract: ABSTRACT [0028] A simple, rapid, and validated UV–visible spectrophotometric method was developed for the quantitative estimation of ascorbic acid and applied to evaluate vitamin C content and antioxidant activity in selected vegetables. Preliminary solubility studies confirmed high aqueous solubility of ascorbic acid, and 3% metaphosphoric acid was selected as the extraction medium due to its stabilizing effect against oxidative degradation. The analytical wavelength was optimized at 265 nm, exhibiting maximum absorbance and minimal interference. The method demonstrated excellent linearity over the concentration range of 10–100 µg/mL with a regression coefficient (R²) of 0.9993, confirming compliance with Beer–Lambert’s law. Validation performed according to ICH Q2(R1) guidelines established satisfactory sensitivity (LOD 0.96 µg/mL; LOQ 2.89 µg/mL), precision (%RSD 2%), accuracy (98–102% recovery), specificity, and robustness. The validated method was successfully applied to quantify vitamin C in various vegetables, revealing the highest content in yellow bell pepper, followed by purple cabbage, while cauliflower showed comparatively lower levels. Antioxidant evaluation using the DPPH assay demonstrated variation in radical scavenging activity among samples, indicating that antioxidant capacity depends not only on vitamin C concentration but also on the presence of other phytochemicals. The developed method provides a reliable, economical, and efficient analytical approach for the routine estimation of ascorbic acid in plant-based food matrices.
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