MUMBAI, India, Aug. 10 -- Intellectual Property India has published a patent application (202511000844 A) filed by Indian Council Of Agricultural Research on January 03, 2025, for Multiplex Polymerase Chain Reaction (pcr) For Species-Level Confirmation Of Major Zoonotic Mycobacterium Tuberculosis Complex (mtbc) Species Of Animal Origin.
Inventors include Santanu Pal; Ravi Kumar Gandham; and Premanshu Dandapat.
The application for the patent was published on July 31, 2026, under issue no. 31/2026.
Abstract: The present invention of one-step sensitive multiplex PCR involved species-specific novel molecular target sequences in various regions of difference (RDs) identified by whole genome analysis and bioinformatic tools to design primers viz., RD1 (Rv3784–Rv3786) for all MTBC except M. bovis BCG; RD4 (Rv1498c–Rv1499) specific for M. tuberculosis and M. orygis; RD4 (Rv1498c – Rv1515c) for confirmation of M. caprae and RD4 (Rv1516c–Rv1517) to identify M. bovis and M. bovis BCG, whereas the RD9-specific primers published earlier were used for confirmation of M. tuberculosis. The multiplex PCR was found to be 100% sensitive and specific for species-level confirmation of MTBC and could detect up to 1.25 pg Mycobacterial DNA, all five MTBC species were successfully detected by this assay down to 12-16 CFU/mg in the spiked lung samples.
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