MUMBAI, India, June 30 -- Intellectual Property India has published a patent application (202617059619 A) filed by Phoenix Biosciences Sa on May 11, 2026, for Methods For Separation And/Or Purification Of Nucleic Acids.
Inventors include Boulanger, Emilie; Dalmat, Sbastien; and Castillo, Jos.
The application for the patent was published on June 26, 2026, under issue no. 26/2026.
Abstract: The current invention relates to a method for nucleic acid purification comprising: (a) combining a sample comprising at least one nucleic acid with a binding buffer having a pH ranging from 4 to 10 and a silica surface, wherein the binding buffer comprises at least one salt and at least one first alcohol, and wherein said salt is present in the combination of the sample and the binding buffer at a concentration ranging from 0.1 M to 5 M, and said at least one first alcohol is present in the combination of the sample and the binding buffer at a concentration between 10% to 50% v/v; (b) incubating the silica surface for a time period sufficient to reversibly bind the at least one nucleic acid to the silica surface creating a nucleic acid-bound silica surface; (c) separating the nucleic acid-bound silica surface from the combined sample and binding buffer; (d) washing the nucleic acid-bound silica surface with at least one wash buffer, wherein said wash buffer comprises at least one second alcohol at a concentration of between 50% to 100%; (e) combining the nucleic acid-bound silica surface with an elution buffer in order to allow the elution of said nucleic acid of interest from said silica surface, wherein said elution buffer has a pH ranging from about 5 to 10 and wherein said elution buffer has a conductivity of between 0.001 and 40 mS/cm or wherein the total salt concentration is from 0 to 50 mM.
Disclaimer: Curated by HT Syndication.
