MUMBAI, India, June 19 -- Intellectual Property India has published a patent application (202618058258 A) filed by Menarini Silicon Biosystems S. P. A. on May 07, 2026, for Method For Analysing The Degree Of Similarity Of At Least Two Samples Using Deterministic Restriction-Site Whole Genome Amplification (drs-Wga).
Inventors include Manaresi, Nicol; Forcato, Claudio; and Ferrarini, Alberto.
The application for the patent was published on June 05, 2026, under issue no. 23/2026.
Abstract: The present disclosure relates to a method for analyzing the degree of similarity of at least two samples in a plurality of samples comprising genomic DNA. The method comprises the following steps. a) Providing a plurality of samples comprising genomic DNA. b) Carrying out, separately on each sample, a deterministic restriction-site whole genome amplification (DRS-WGA) of said genomic DNA, c) Preparing a massively parallel sequencing library using a fragmentation-free, sequencing- adaptor/WGA fusion-primer PCR reaction from each product of said DRS-WGA. d) Carrying out low-pass whole genome sequencing at a mean coverage depth of 1x on said massively parallel sequencing library. e) Aligning for each sample the reads obtained in step d) on a reference genome. f) Extracting for each sample the allelic content at a plurality of polymorphic loci. g) Calculating a pair-wise similarity score for the at least two samples as a function of the allelic content measured at said plurality of loci. h) Determining the degree of similarity of the at least two samples on the basis of the similarity score. [Fig. 1]
Disclaimer: Curated by HT Syndication.
