Intellectual Property India Publishes Patent Application for Genetically Expanded T7-Rna-Polymerase (ssbt7-Rnap): Fluorescent Triazolyl Cyanobenzene Alanine (tcnbalaac) Unnatural Amino Acid At Y571 Codon Site

MUMBAI, India, July 13 -- Intellectual Property India has published a patent application (202531019332 A) filed by Indian Institute Of Technology Guwahati; and Prof. Dr. Subhendu Sekhar Bag on March 04, 2025, for Genetically Expanded T7-Rna-Polymerase (ssbt7-Rnap): Fluorescent Triazolyl Cyanobenzene Alanine (tcnbalaac) Unnatural Amino Acid At Y571 Codon Site.

Inventors include Prof. Dr. Subhendu Sekhar Bag; and Aniket Banerjee.

The application for the patent was published on July 10, 2026, under issue no. 28/2026.

Abstract: ABSTRACT GENETICALLY EXPANDED T7-RNA-POLYMERASE (SSBT7-RNAP): FLUORESCENT TRIAZOLYL CYANOBENZENE ALANINE (TCNBALAAC) UNNATURAL AMINO ACID AT Y571 CODON SITE The invention relates to the site-specific incorporation of a novel fluorescent triazolyl unnatural amino acid (FTUAA) into T7 RNA polymerase using genetic code expansion (GCE) techniques, more particularly, it address one critical site is the Y571 position, a mutation hotspot adjacent to the enzyme’s active site, where substitutions such as Y571S (tyrosine to serine) typically lead to compromised activity and lower transcriptional efficiency. Figure: 1

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