MUMBAI, India, June 22 -- Intellectual Property India has published a patent application (202511032136 A) filed by Bharat Petroleum Corporation Limited; and International Centre For Genetic Engineering And Biotechnology on March 31, 2025, for Genetically Engineered Microbial Construct And Method Of Producing 1,4-Butanediol Thereof.
Inventors include Madan, Bhawna; Rathore, Vivek; Rawat, Jaya; Yazdani, Syed Shams; Dev, Chandra; Manzer, Abdur Rahman; and Gupta, Payal.
The application for the patent was published on June 12, 2026, under issue no. 24/2026.
Abstract: The present invention relates to a genetically engineered host cell optimized for high-yield biosynthesis of 1,4-butanediol (BDO) through stable genomic integration of key biosynthetic pathway genes. The invention eliminates plasmid dependency by integrating seven codon-optimized heterologous genes (cat1, sucD, 4hbd, cat2, bld, bdh, sucA) at strategic genomic loci in the bacterial host. Deletion of competing metabolic pathways enhances carbon flux redirection toward BDO production. A T5 promoter with IPTG induction fine-tunes metabolic expression, reducing byproducts and improving yield. Optimized fermentation conditions (pH, temperature, microaerobic control) and in situ product recovery (ISPR) further enhance BDO productivity. The invention enables cost-effective, bio-based BDO production from renewable feedstocks including sugars derived from plants such as glucose, xylose, arabinose, galactose, lactose, glycerol, and lignocellulosic hydrolysates, offering an eco-friendly alternative to petrochemical synthesis. This scalable microbial platform is suitable for applications in bioplastics, solvents, fibers, and biofuels, making it a key innovation in industrial biotechnology.
Disclaimer: Curated by HT Syndication.
