MUMBAI, India, Aug. 17 -- Intellectual Property India has published a patent application (202641065844 A) filed by Manipal Academy Of Higher Education on May 25, 2026, for Engineered Nucleic Acid And Vector For Mirna Cluster Overexpression In Mammalian Cells, And Uses Thereof.
Inventors include Roopal Behera; Pratheeksha Hebbar; Alfa Florence Rodrigues; Samatha Bhat; and Shama Prasada K.
The application for the patent was published on August 14, 2026, under issue no. 33/2026.
Abstract: The present disclosure relates to engineered nucleic acid sequences and recombinant vector constructs for coordinated overexpression of a miR-127/136 microRNA (miRNA) cluster. The engineered sequence comprises multiple miRNA stem-loop sequences arranged in a defined, non-naturally occurring configuration within a single contiguous nucleic acid sequence, optionally separated by spacer sequences. The construct is incorporated into an expression vector comprising regulatory elements including a promoter and a selectable marker. Methods are disclosed for introducing the construct into mammalian cells via viral or non-viral delivery systems and selecting stable cell lines expressing the miRNA cluster. The disclosure further provides engineered cell lines, screening platforms, and kits useful for functional studies of miRNA-mediated gene regulation and cancer research applications.
Disclaimer: Curated by HT Syndication.
