MUMBAI, India, Oct. 5 -- Intellectual Property India has published a patent application (202641115233 A) filed by Dr. B. Ramya Kuber on September 25, 2026, for Comprehensive Green Assessment Metrics Driven : A Novel Uplc Method For The Estimation Of Methenamine Hippurate.
Inventors include Dr. B. Ramya Kuber; Yadlapalli Tejaswini; Jalla. Jyothirmayi; B. Charishma Naga Sai; Panasa Meghana; K. Thilothama; Madasu Bhargavi; B. Saanvi Sri; and B. Dheeraj.
The application for the patent was published on October 02, 2026, under issue no. 40/2026.
Abstract: ABSTRACT [0014] A novel stability-indicating ultra-performance liquid chromatographic method has been developed and fully validated for the accurate quantitative determination of Methenamine Hippurate in bulk powder and in tablet dosage forms. The method employs a Waters Acquity UPLC system equipped with a high- strength silica column of dimensions 100 mm by 2.1 mm packed with 1.7 micrometre particles. Separation is achieved under isocratic conditions using a mobile phase consisting of methanol and 0.1 percent orthophosphoric acid in the volumetric ratio of 40 to 60, delivered at a flow rate of 0.3 millilitres per minute. Detection is performed at a wavelength of 228 nanometres with the column maintained at 30 degrees Celsius and an injection volume of 1 microlitre. Under these conditions the analyte elutes at approximately 1.05 minutes with excellent peak symmetry and column efficiency. [0015] The method has been validated according to current international guidelines and demonstrates linearity over the concentration range of 10 to 60 micrograms per millilitre with a correlation coefficient of 0.9995. Accuracy, expressed as mean recovery, falls between 99.05 and 99.64 percent. Precision, evaluated as both repeatability and intermediate precision, yields relative standard deviations below 0.5 percent. Limits of detection and quantification are 0.26 and 0.79 micrograms per millilitre respectively. Forced degradation studies conducted under acid, base, peroxide, thermal, photolytic and neutral conditions confirm that the analyte peak remains well resolved from all degradation products, thereby establishing the stability-indicating nature of the procedure. Environmental sustainability of the method has been assessed using the AGREE metric, confirming a favourable green profile arising from reduced solvent consumption, short analysis time and minimal waste generation. The method is therefore suitable for routine quality-control analysis and stability testing of Methenamine Hippurate formulations.
Disclaimer: Curated by HT Syndication.
