MUMBAI, India, Nov. 2 -- Intellectual Property India has published a patent application (202611103167 A) filed by Indian Council Of Agricultural Research on August 26, 2026, for A Serum-Free Medium For Culturing Buffalo Embryos And Method Of Preparation Thereof.
Inventors include Dharmendra Kumar; Meeti Punetha; Manu Mangal; and Pradeep Kumar.
The application for the patent was published on October 30, 2026, under issue no. 44/2026.
Abstract: The present disclosure relates to a buffalo-specific, serum-free, single-step medium for in vitro culture of pre-implantation buffalo embryos. The medium comprises a defined basal media containing trisodium citrate and myo-inositol; physiologically calibrated glucose, lactate and pyruvate; essential and non-essential amino acids with L-glutamine; serum replacer, sericin and polyvinyl alcohol; N-acetyl-5-methoxytryptamine and insulin-transferrin-selenium; epidermal growth factor; and an antimicrobial supplement. The medium supports continuous culture from the post-fertilization stage to the blastocyst stage without serum or a somatic-cell feeder layer. The disclosure further provides methods and uses of the medium in in vitro fertilization, ovum pick-up, parthenogenetic activation and somatic cell nuclear transfer embryo systems.
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