MUMBAI, India, Aug. 31 -- Intellectual Property India has published a patent application (202641101367 A) filed by National Institute Of Technology-Warangal on August 21, 2026, for A Scalable Bioprocess For Sustainable Production Of Citramalic Acid.
Inventors include Dr. Ashish A Prabhu; and Satwika Das.
The application for the patent was published on August 28, 2026, under issue no. 35/2026.
Abstract: Title: A Scalable Bioprocess for Sustainable Production of Citramalic Acid. The present invention relates to a process for producing citramalic acid using a genetically modified yeast strain and food waste hydrolysate-derived carbon sources. The process comprises introducing an exogenous codon-optimized citramalate synthase gene, a mitochondrial tricarboxylate transporter gene, and a citramalate export transporter gene into a yeast strain to enhance citramalate biosynthesis and secretion. The genetically modified yeast strain is cultivated in a fermentation medium comprising food waste hydrolysate-derived glucose, nitrogen sources, mineral salts, trace elements, and optional secondary carbon sources in a batch bioreactor under optimized fermentation conditions. The citramalate synthase catalyses the formation of citramalate from pyruvate and acetyl-CoA, while the transporter proteins improve intracellular carbon flux and extracellular citramalate export. Medium composition and process parameters are optimized using plackett-burman design, central composite design, artificial neural network modelling, and genetic algorithm optimization. The process provides a sustainable and economically viable platform for producing citramalic acid as a renewable precursor for methyl methacrylate, polymethyl methacrylate, and other industrial chemicals.
Disclaimer: Curated by HT Syndication.
