MUMBAI, India, July 31 -- Intellectual Property India has published a patent application (202411102373 A) filed by Council Of Scientific And Industrial Research An Indian Registered Body Incorporated Under The Regn. Of Soc. Act Act Xxi Of on December 23, 2024, for A Recombinant Vector Useful For Endosperm-Specific Genome Editing In Rice.
Inventors include Channakeshavaiah Chikaputtaiah; Dhanawantari Lourembam Singha; Ajay Kumar Keot; and Natarajan Velmurugan.
The application for the patent was published on July 24, 2026, under issue no. 30/2026.
Abstract: The present invention relates to a recombinant vector for genome editing in rice. The vector comprises of GluB1 promoter of rice along with Cas 12 a gene and a guide RNA. Editing the targeted gene within localized tissue effectively mitigates the pleiotropic effect observed in non-targeted tissues, thus reducing the potential for unintended consequences. Tissue-specific genome editing (TSGE) plays a pivotal role in alleviating the metabolic burden on plants by restricting modifications to specific areas. The designed CRISPR-ESGE binary construct was introduced into Agrobacterium, which was then used to infect rice callus derived from seeds. The regenerated transformed plants were confirmed to contain the Cas12a gene, and the endosperm-specific expression of Cas12a was validated through RT-PCR analysis. Genome editing (GE) utilizing Cas12a offers distinct advantages over Cas9, which is characterized by heightened target specificity and decreased propensity for off- target effect, thereby potentially enhancing the grain starch content.
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