MUMBAI, India, Sept. 28 -- Intellectual Property India has published a patent application (202511016560 A) filed by Indian Council Of Agricultural Research on February 25, 2025, for A Recombinant Crispr/Cas9 System For Genome Editing In Pigeon Pea.
Inventors include Sreevathsa, Rohini; Senthil, Kameshwaran; Rathinam, Maniraj; Parashar, Manisha; Dokka, Narasimham; Tyagi, Shaily; Mathur, Vandana; Sharma, Sandhya; Gaikwad, Kishor; and Bhattacharya, Ramcharan.
The application for the patent was published on September 25, 2026, under issue no. 39/2026.
Abstract: Pigeon pea is an important legume valued for its high nutritional, agricultural, and economic significance. Despite its potential for high yield, productivity remains stagnant due to several abiotic and biotic stresses. To mitigate these challenges, biotechnological interventions like genome editing offer promising solutions. Towards this, developing a species-specific editing toolkit is crucial for recalcitrant species like pigeon pea. The present disclosure relates to development of a CRISPR/Cas9 genome editing system targeting the phytoene desaturase (PDS) gene. The present disclosure provides pigeon pea-compatible vector components, including the CcU6_7.1 promoter and an amenable Cas9 gene driven by the potato ubiquitin promoter, creating a pigeon pea-specific CRISPR/Cas9 binary vector (PP_CRISPR_pCAMBIA2301). The system was validated by Agrobacterium tumefaciens-mediated apical meristem-targeted in planta and in vitro embryonic axis explant transformations, with gene knockout confirmed by albino/bleached phenotypes. This system may support functional genomics studies and trait improvement in pigeon pea and other legumes.
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