MUMBAI, India, Oct. 26 -- Intellectual Property India has published a patent application (202611107877 A) filed by Maharishi Markandeshwar Deemed To Be University on September 08, 2026, for A Novel First-Order Derivative Uv Spectrophotometric Method For The Simultaneous Quantification Of Rutin And Ursolic Acid In Marketed Green Tea Formulations.
Inventors include Prof. Dr. Rina Das; Prof. Dr. Dinesh Kumar Mehta; Jaspreet Kaur; Devashish Upadhyay; and Ayan Aggarwal.
The application for the patent was published on October 23, 2026, under issue no. 43/2026.
Abstract: The present invention relates to a first-order derivative UV spectrophotometric method for the simultaneous quantification of Rutin and Ursolic acid in marketed green tea formulations. The method employs methanol as a diluent and utilizes zero-crossing first-order derivative spectrophotometry to minimize spectral interference between the analytes without requiring chemical separation. The method comprises preparing and extracting the green tea sample using methanol and ultrasonic treatment, recording UV spectra in the range of 200–400 nm, and converting the spectra into first-order derivative spectra. Rutin is quantified at about 374 nm, where Ursolic acid exhibits zero derivative amplitude, while Ursolic acid is quantified at about 300.5 nm, where Rutin exhibits zero derivative amplitude. Calibration is performed over 4–24 µg/mL. The method provides a simple, rapid, precise, accurate and cost-effective approach for quantitative analysis and standardization of marketed green tea formulations.
Disclaimer: Curated by HT Syndication.
