MUMBAI, India, July 24 -- Intellectual Property India has published a patent application (202611062014 A) filed by Indian Council Of Agricultural Research on May 15, 2026, for A Molecular Method For Determining Rootstock Species In Grafted Nutmeg Plants.
Inventors include Muhammed Azharudheen Thazheyil Pura; Muhammed Nissar Vettathukattil Abdul; Sharon Aravind; and Sheeja Thotten Elampilay.
The application for the patent was published on July 17, 2026, under issue no. 29/2026.
Abstract: The present disclosure relates to a method for determining a rootstock species in a grafted nutmeg plant. The method comprises collecting a bark tissue sample from a rootstock, extracting genomic DNA, and amplifying a chloroplast genomic region comprising a ycf4_cemA intergenic spacer using a pair of oligonucleotide primers. The method further comprises determining a size of at least one amplified DNA fragment, wherein an amplified fragment of about 164 base pairs indicates Myristica fragrans, and an amplified fragment greater than 164 base pairs indicates a wild Myristica species. The method is based on detection of a species-specific deletion polymorphism in the target region. The disclosure further provides primer sets and a diagnostic kit for performing the method. The approach enables rapid, reliable, and cost-effective identification of rootstock species at an early stage using minimal tissue input and without requiring sequencing.
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