MUMBAI, India, July 7 -- Intellectual Property India has published a patent application (202541000272 A) filed by Kcat Enzymatic Private Limited. on January 02, 2025, for A Method Of Preparation Of Tyrian Purple Using Engineered Enzyme Cascades.
Inventors include Pravin Kumar. R; Gladstone Sigamani. G; Roopa. L; Anuj. J Shetty; Abhishek Kumar; Likith M; Naveen B K; and Tharun Bharadwaj L.
The application for the patent was published on July 03, 2026, under issue no. 27/2026.
Abstract: A METHOD OF PREPARATION OF TYRIAN PURPLE USING ENGINEERED ENZYME CASCADES: Tyrian purple, mainly composed of 6,6'-dibromoindigo, is an ancient dye extracted from sea snails. However, its synthesis remains limited due to uncharacterized biosynthetic pathway and the difficulty of regiospecific bromination. Here, we introduce an effective 6,6'- dibromoindigo production strategy in E. coli using halogenase to convert natural plant derived indigo to 6,6?-dibromoindigo. A single cell system was designed to overproduce regiospecifically brominated Tyrian purple. This approach led to an improved production of 6,6'-dibromoindigo from indigo as a substrate with a successful biosynthesis of regiospecifically dihalogenated Indigo. Furthermore, it was demonstrated that 6,6'-dibromoindigo converting cells can be directly used as a bacterial dye.
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