MUMBAI, India, Aug. 12 -- Intellectual Property India has published a patent application (202421065702 A) filed by Amity University Madhya Pradesh on August 30, 2024, for A Method For Enhancing Secondary Metabolites In Catharanthus Roseus Using Electric Field- Assisted Suspension Culture.
Inventors include Raghvendra Kumar Mishra; Anamika; Neha Sharma; and Vinay Dwivedi.
The application for the patent was published on August 07, 2026, under issue no. 32/2026.
Abstract: The present invention relates to a method for enhancing the production of secondary metabolites in Catharanthus roseus through electric field-assisted suspension culture. The method comprises sterilizing seeds, germinating them on Murashige and Skoog (MS) medium, preparing leaf explants, and subjecting the explants to a sequential sterilization process involving washing under running tap water, treatment with Tween 20, ethanol immersion, and mercuric chloride sterilization. Callus is induced on MS medium supplemented with 6-Benzylaminopurine (BAP) and a-Naphthalene acetic acid (NAA), followed by establishment of suspension cultures under agitation. The suspension cultures are then exposed to an external electric field ranging from 6V to 10V, which significantly enhances the accumulation of secondary metabolites including alkaloids, phenols, and flavonoids compared to untreated controls. The invention provides a novel and effective protocol to improve the nutritional and pharmaceutical value of Catharanthus roseus extracts, with potential applications in nutraceuticals, pharmaceuticals, and antioxidant formulations. Accompanied Drawing [Figure 1]
Disclaimer: Curated by HT Syndication.
