MUMBAI, India, Aug. 31 -- Intellectual Property India has published a patent application (202631101557 A) filed by Institute Of Engineering And Management - School Of University Of Engineering And Management on August 22, 2026, for A Cost-Effective Kit-Based Formulation And Protocol For Dna Extraction From Microbial Biofilms.
Inventors include Dr. Sohini Sen; Dr. Dibyajit Lahiri; Dr. Moupriya Nag; Dr. Debasmita Bhattacharya; Ms. Debashrita Majumder; Dr. Rupsa Bhattacharya; and Mr. Avijit Chakraborty.
The application for the patent was published on August 28, 2026, under issue no. 35/2026.
Abstract: The present invention is a low-cost, kit-based method and formulation for extracting deoxyribonucleic acid (DNA) from bacterial cells and microbial biofilms, including both Gram-positive and Gram-negative bacteria. For cell resuspension, enzyme inhibition, cellular disruption, contaminant separation, and DNA precipitation, the process uses Tris-EDTA buffer, sodium dodecyl sulfate, Proteinase K, phenol-chloroform, sodium acetate, and isopropanol. For use in subsequent molecular biology applications, the technique effectively recovers genomic DNA while providing DNA having suitable yield and purity for subsequent molecular biology applications. Microbial cells are cultured, harvested and resuspended, treated with specific reagents, phase separated, precipitated, washed, and resuspended, and then the concentration and purity of DNA are measured using spectrophotometry. Biofilm, bacterial, oral, and dental swab samples, as well as associated genetic analyzes, can all be performed with this kit.
Disclaimer: Curated by HT Syndication.
