MUMBAI, India, Oct. 26 -- Intellectual Property India has published a patent application (202611011432 A) filed by Deen Dayal Upadhyaya Gorakhpur University on February 03, 2026, for A Blood-Free Culture Medium Lysinibacillus Fast-Select Medium (lfsm) For Rapid Isolation, Enrichment, And Differentiation Of Bacteria.
Inventors include Priyanka Bharti; and Sarad Kumar Mishra.
The application for the patent was published on October 23, 2026, under issue no. 43/2026.
Abstract: The present invention relates to a novel, blood-free selective and enriched culture medium designated Lysinibacillus Fast-Select Medium (LFSM) for rapid isolation, enrichment, and differentiation of bacteria belonging to the genus Lysinibacillus and its method of preparation. The medium comprises plant-derived peptones, yeast extract, a fermentable carbon source, elevated sodium chloride, trace boron, osmoprotectants, surfactants, and a redox indicator, optionally solidified with agar. LFSM selectively supports halotolerant and boron-tolerant Lysinibacillus species while suppressing competing bacteria and fungi, without the use of animal blood or serum. The inclusion of glycine betaine and Tween 80 enhances growth under osmotic stress, while 2,3,5-triphenyltetrazolium chloride enables visual differentiation of viable and sporulating colonies. The medium provides higher recovery rates, faster sporulation, and significant cost reduction compared to conventional blood-based or non- selective media, making it suitable for environmental, agricultural, industrial, and clinical microbiology applications.
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