MUMBAI, India, Nov. 21 -- Intellectual Property India has published a patent application (202517097557 A) filed by National University Corporation Hokkaido University; and Cellspect Co. Ltd., Hokkaido, Japan, on Oct. 9, for 'microbial nucleic acid detection method, reagent composition, reagent kit, measurement system, and program.'
Inventor(s) include Satoh Hisashi; Nakaya Yuki; Nakajima Meri; Handa Hisazumi; Sanari Kota; Hirano Reiko; Doi Kazuhiko; Hojo Wataru; Iwabuchi Takuya; and Sakai Hironori.
The application for the patent was published on Nov. 21, under issue no. 47/2025.
According to the abstract released by the Intellectual Property India: "Provided is a microbial nucleic acid detection method, etc., that permits fast, easy, and highly accurate measurement of the concentration of a target nucleic acid and can also ensure an adequate measurement range (dynamic range). The microbial nucleic acid detection method comprises: a step in which a probe solution, which is a solution of a gold nanoparticle probe in which DNA that binds specifically to the target nucleic acid is modified with gold nanoparticles, is mixed with an extract obtained by subjecting a sample to nucleic acid extraction treatment, sodium chloride, at least, is also added and the mixture is heated to prepare a sample mixed solution; a step in which an absorption spectrum of the probe solution is acquired; a step in which an absorption spectrum of a blank solution is acquired, the blank solution being is a sample-free solution obtained by adding sodium chloride to the probe solution and heating; a step in which an absorption spectrum of the sample mixed solution is acquired; and a calculation step in which the concentration of the target nucleic acid in the sample is estimated based on the absorption spectra of the probe solution, the blank solution, and the sample mixed solution."
The patent application was internationally filed on Apr. 18, 2024, under International application No.PCT/JP2024/015385.
Disclaimer: Curated by HT Syndication.
