MUMBAI, India, Feb. 6 -- Intellectual Property India has published a patent application (202511134325 A) filed by Maharishi Markandeshwar, Ambala, Haryana, on Dec. 31, 2025, for 'lateral flow immunoassay device for detecting apple stem grooving virus (asgv) and apple mosaic virus (apmv).'
Inventor(s) include Anchal Saini; Gourav Vats; Yashika Walia Dhir; and Sunny Dhir.
The application for the patent was published on Feb. 6, under issue no. 06/2026.
According to the abstract released by the Intellectual Property India: "The invention discloses a lateral flow immunoassay (LFIA) device for rapid, field-level detection of apple stem grooving virus (ASGV) and apple mosaic virus (ApMV). The device comprises a sample pad for receiving plant extracts, a conjugate pad impregnated with gold nanoparticle-antibody (AuNP-Ab) conjugates specific to ASGV and ApMV, a nitrocellulose membrane (NCM) incorporating a test line coated with virus-specific antibodies and a control line coated with goat anti-rabbit IgG, and an absorbent pad to maintain capillary flow. The AuNP-Ab conjugates, prepared at optimal antibody concentrations of 4-6 g and conjugated at pH 7, generate a visible colorimetric signal upon antigen-antibody interaction. The device delivers results within minutes, producing two distinct bands for positive samples and a single control band for negatives. Storage formulations comprising bovine serum albumin (BSA) and trehalose enhance stability. The LFIA device is portable, cost-effective, and user-friendly, enabling accurate detection of ASGV and ApMV in remote apple-growing regions without laboratory infrastructure."
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