MUMBAI, India, Feb. 13 -- Intellectual Property India has published a patent application (202611000859 A) filed by Nims University, Jaipur, Rajasthan, on Jan. 5, for 'immunoassay pad and method for analyte detection.'

Inventor(s) include Gayatri Pandey; Akshat Pratap Singh; and Vaibhav Tamrakar.

The application for the patent was published on Feb. 13, under issue no. 07/2026.

According to the abstract released by the Intellectual Property India: "An immunoassay pad (100) for ultra-sensitive in vitro detection and quantification of an analyte in biological, environmental or food samples. The immunoassay pad comprises a support pad (101), a nitrocellulose or polymeric membrane, having a primary capture antibody (101a) immobilized thereon, and a suspension of functionalized microbeads (102), each carrying a secondary detection antibody (102a) and an enzyme label (102b). A chromogenic substrate (103) reacts with enzyme to produce discrete coloured dots, each representing a single binding event of an analyte. The pad is imaged using digital imaging device (104), and then a machine learning based automated image analysis system is used to precisely count the dots and correlate the count with the analyte concentration. The method (200) involves a set of sequential steps, including sample application, binding of the microbeads, washing, reaction with substrate, imaging, and quantitative analysis, to provide fast, reproducible, and ultra-sensitive detection of low-level analytes."

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