MUMBAI, India, April 10 -- Intellectual Property India has published a patent application (202441075459 A) filed by Yenepoya, Mangalore, Karnataka, on Oct. 5, 2024, for 'herbal formulation for wound healing.'
Inventor(s) include Chandrashekhara Kumar B; Prajitha Biju; Arun A Bhagwath; and Mohammed Gulzar Ahmed.
The application for the patent was published on April 10, under issue no. 15/2026.
According to the abstract released by the Intellectual Property India: "I/We claim: 1) An herbal formulation for wound healing comprising: a) herbal extracts; b) hydrogel-forming polymer; c) penetration enhancer; d) pH regulator; and e) preservative(s). 2) The formulation as claimed in claim 1, wherein the herbal extracts are selected from bark of Acacia catechu, gall of Quercus infectoria, leaves of Chromolaena odorata and fruit of Terminalia chebula; wherein the 100 mL of formulation comprises of 250g/ml of phytochemicals from Terminalia chebula, 250 g/ml of phytochemicals from Quercus infectoria, 100g/ml of phytochemicals from Acacia catechu, and 250g/ml of phytochemicals from Chromolaena odorata. 3) The formulation as claimed in claim 2, wherein the bark extract of Acacia catechu comprises of phytochemicals including alkaloids, flavonoids, tannins, phenolic compounds and phytosterols; wherein the fruit extract of Terminalia chebula comprises of phytochemicals including flavonoids, tannins, glycosides, carbohydrates, and phenolic compounds; wherein the gall extract of Quercus infectoria comprises of phytochemicals including flavonoids, tannins, diterpenes, triterpenoids, carbohydrates and phytosterols; wherein the leaf extract of Chromolaena odorata comprises of phytochemicals including alkaloids, flavonoids, tannins, diterpenes, triterpenoids, saponins, carbohydrates, and phenolic compounds. 4) The formulation as claimed in claim 1, wherein the hydrogel-forming polymer is carbopol 980; wherein the penetration enhancer is glycerol; wherein the pH regulator is triethanolamine; wherein the preservative is benzyl alcohol. 5) The formulation as claimed in claim 1, wherein the formulation has a pH of 6.5, viscosity of 1350 cP, and an extrudability of 0.15g/N. 6) The formulation as claimed in claim 1, wherein the formulation exhibits antibacterial activity against Staphylococcus aureus with a zone of inhibition of 21-24 mm, Bacillus subtilis with a zone of inhibition of 19-22 nm, Escherichia coli with a zone of inhibition of 17-20 nm, Pseudomonas aeruginosa with a zone of inhibition of 15-18 mm; wherein the formulation exhibits antifungal activity against Candida albicans with a zone of inhibition of 18-21 mm, Aspergillus niger with a zone of inhibition of 16-19 mm, and Fusarium oxysporum with a zone of inhibition of 14-17 mm. 7) The formulation as claimed in claim 1, wherein the formulation exhibits an astringency index of 48 3 mg TAE/g. 8) The formulation as claimed in claim 1, wherein the formulation at 1 mg/mL shows haemolysis at 1.2 0.4% and at 5 mg/mL shows haemolysis at 2.8 0.6%. 9) The formulation as claimed in claim 1, wherein the formulation shows an average clotting time of 3.0 0.2 minutes and reduced the blood clotting time by 42% compared to control. 10) The formulation as claimed in claim 2, wherein the formulation exhibits an anti-inflammatory activity, wherein Acacia catechu exhibits an inhibition of 85.08% at 400 g/mL; wherein Terminalia chebula exhibits an inhibition of 50.62% inhibition at 400 g/mL; wherein Quercus infectoria exhibits an inhibition of 89.53% at 400 g/mL; wherein Chromolaena odorata exhibits an inhibition of 76.75% at 400 g/mL; wherein Diclofenac Sodium (control) exhibits an inhibition of 77.45% at 400 g/mL. 11) The formulation as claimed in claim 2, wherein the formulation exhibits an antioxidant activity, wherein Acacia catechu shows 65-70% inhibition at 250 g/mL, Chromolaena odorata shows 60-65% inhibition at 250 g/mL, Quercus infectoria shows 40-45% inhibition at 250 g/mL, and Terminalia chebula shows 20-25% at 250 g/mL. 12) The formulation as claimed in claim 1, wherein the formulation shows a Limit of Quantification (LOQ) of =0.01 mg/kg for heavy metals including As, Pb, Cd, and Hg; wherein the formulation shows a LOQ of =0.5 g/kg for alfatoxin. 13) The formulation as claimed in claim 1, wherein the formulation shows a LOQ of =0.01 mg/kg for organochlorine pesticides including DDT, aldrin, dieldrin, heptachlor; wherein the formulation shows a LOQ of =0.1 mg/kg per analyte for organophosphates, carbamates, pyrethroids, neonicotinoids; wherein the formulation shows a LOQ of =0.1 mg/kg for fungicides and herbicides including glyphosate, atrazine, triazoles, and strobilurins; wherein the formulation shows a LOQ of =0.05 mg/kg for acaricides, nematicides, rodenticides, bactericides; wherein the formulation has a total pesticide content of =0.5 mg/kg. 14) The formulation as claimed in claim 2, wherein Acacia catechu exhibits cell exhibits cell migration ability at the end of 30 hours, with 84.31%; wherein Terminalia chebula exhibits cell migration ability at the end of 30 hours, with 96.98% scratch closure; wherein Chromolaena odorata exhibits cell migration ability at the end of 30 hours with 84.74 %; wherein Quercus infectoria exhibits cell migration ability at the end of 30 hours with 84.80%. 15) The formulation as claimed in claim 2, wherein Acacia catechu exhibits an increase in cell proliferation from 100.44% at 25 g/mL to 111.96 % at 250 g/mL; wherein Quercus infectoria exhibits an increase in cell proliferation from 105.34% at 25 g/mL to 123.62 1.54% at 250 g/mL; wherein Terminalia chebula exhibits an increase in cell proliferation from 109.78% at 25 g/mL to 132.83 1.26% at 250 g/mL; wherein Chromolaena odorata exhibits an increase in cell proliferation from 100.10 % at 25 g/mL to 111.93 % at 250 g/mL. 16) A method for preparation of herbal formulation, wherein the method comprises steps of: a) collecting herbs comprising bark of Acacia catechu, gall of Quercus infectoria, leaves of Chromolaena odorata and fruit of Terminalia chebula; shade-drying each plant material for 5-7 days and powdering four herbs using a mechanical grinder; b) subjecting the powdered samples from step (b) to soxhlet extraction for 6-8 hours; c) concentrating the extracts by using rotary evaporator at 40-45 C; drying the concentrated extracts in vacuum desiccator; obtaining the herbal extracts; d) weighing 0.5-2% of carbopol 980 and soaking in 80 mL of water for 20 minutes; neutralizing until a clear solution is formed, with stirrer and pH detector; e) adding 0.001-1% glycerol into the mixture obtained from step (d); f) neutralizing swollen carbopol- glycerol solution from step (e) with 0.5 % triethanolamine (TEA) dropwise with continuous stirring, until a clear, transparent gel is formed; maintaining the pH in the range of 6.0-7.0; g) incorporating 0.25% of gall extract of Quercus infectoria, 0.25% bark extract of Acacia catechu, 0.25% fruit extract of Terminalia chebula, and 0.25% leaf extract of Chromolaena odorata into the gel mixture from step (f); stirring continuously under controlled temperature at 25-30 C to ensure uniform dispersion of extracts; h) adding 0.5-1% of benzyl alcohol to the mixture from step (g); and making up the final volume to 100.0 mL by stirring continuously until a homogenous gel is obtained. 17) An herbal formulation for targeting superficial wounds, second degree burns, and open wounds comprising: a) herbal extracts; b) hydrogel-forming polymer; c) penetration enhancer; d) pH regulator; and e) preservative(s). 18) The formulation as claimed in claim 17, wherein the herbal extracts are selected from bark of Acacia catechu, gall of Quercus infectoria, leaves of Chromolaena odorata and fruit of Terminalia chebula; wherein the hydrogel-forming polymer is carbopol 980; wherein the penetration enhancer is glycerol; wherein the pH regulator is triethanolamine; wherein the preservative is benzyl alcohol. Dated this 6th October, 2025 (LIPIKA SAHOO) Registration Number: IN/PA-2467 Agent for Applicant This document is signed with the digital signature of Patent Agent for the Applicant LIPIKA SAHOO (IN/PA-2467)."
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