MUMBAI, India, Feb. 6 -- Intellectual Property India has published a patent application (202511134328 A) filed by Maharishi Markandeshwar, Ambala, Haryana, on Dec. 31, 2025, for 'development of dual immunodiagnostics for rapid detection of apple necrotic mosaic virus in plant samples.'
Inventor(s) include Gourav Vats; Anchal Saini; Yashika Walia Dhir; and Sunny Dhir.
The application for the patent was published on Feb. 6, under issue no. 06/2026.
According to the abstract released by the Intellectual Property India: "The invention discloses a dual immunodiagnostic system for the rapid detection of apple necrotic mosaic virus (ApNMV) in plant samples. Recombinant coat protein of ApNMV was cloned, expressed, and purified to generate specific polyclonal antibodies. These antibodies were validated by indirect enzyme-linked immunosorbent assay (ELISA) for laboratory-based detection and conjugated to colloidal gold nanoparticles for lateral flow immunoassay (LFIA) strip assembly. The indirect ELISA provides reliable mass screening through antigen-antibody binding, producing measurable absorbance at 405 nm. The LFIA device enables field-level detection, with antibody-conjugated nanoparticles producing distinct test and control lines on nitrocellulose membranes within minutes. Optimization studies confirmed antibody concentrations of 10-12 g/mL provided maximum nanoparticle stability, preventing salt-induced aggregation. Validation against RT-PCR demonstrated 100% sensitivity and specificity for LFIA, while ELISA exhibited 70% diagnostic sensitivity. The invention thus provides a cost-effective, portable, and user-friendly diagnostic solution for farmers, nurseries, and plant health agencies to manage ApNMV infections and reduce economic losses."
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