MUMBAI, India, Feb. 13 -- Intellectual Property India has published a patent application (202611001255 A) filed by Maharishi Markandeshwar, Ambala, Haryana, on Jan. 6, for 'a time-dependent in-vitro evaluation of antimicrobial activity in pharmaceutical formulations.'
Inventor(s) include Anuj Malik; Uditi Handa; and Kumar Gurave.
The application for the patent was published on Feb. 13, under issue no. 07/2026.
According to the abstract released by the Intellectual Property India: "The present invention discloses a novel time phased in vitro evaluation model for assessing antimicrobial activity in pharmaceutical formulations, particularly sustained release lozenges. Conventional antimicrobial assays fail to capture dynamic drug release and microbial inhibition over time, limiting their relevance to real therapeutic conditions. To overcome these limitations, the Sequential Time Point Diffusion Assay was developed, integrating agar well diffusion methodology with temporal monitoring. Formulations containing 1%, 3%, and 5% w/w active pharmaceutical ingredient (API) were compared against a marketed standard. Inhibition zones were recorded at sequential intervals of 1, 2, 4, 8, 24, 48, 72, and 96 hours, and a novel quantitative index, the Antimicrobial Activity Factor (AMF), was calculated to characterize consistency and potency of antimicrobial action. Results demonstrated concentration dependent and time dependent efficacy, with higher API formulations exhibiting earlier onset and stronger inhibition. Batch B4 (5%) showed rapid burst release activity with a peak inhibition zone of 11.2 cm at 8 hours, while Batch B3 (3%) exhibited sustained release with peak activity at 24 hours. The marketed product displayed delayed release kinetics, peaking at 48 hours. This reproducible model provides a robust framework for evaluating time resolved antimicrobial efficacy, supporting rational development, optimization, and regulatory assessment of sustained release antimicrobial formulations."
Disclaimer: Curated by HT Syndication.
