MUMBAI, India, April 10 -- Intellectual Property India has published a patent application (202421076264 A) filed by Indian Oil Corporation Limited, Maharashtra, on Oct. 8, 2024, for 'a system and assay for on-site detection and quantification of corrosion causing microbes.'

Inventor(s) include Sahoo, Prakash Chandra; Kumar, Manoj; Pragya; Gupta, Ravi Prakash; and Srivastav, Umish.

The application for the patent was published on April 10, under issue no. 15/2026.

According to the abstract released by the Intellectual Property India: "The present disclosure relates to a real-time polymerase chain reaction (RT-PCR) based system and assay for rapid and accurate on-site detection of corrosion-causing microbes in diverse industrial settings. The present disclosure provides RT-PCR-reagents comprising (a) sample agnostic DNA isolation bio-buffer (b) PCR augmenter and (c) PCR reaction medium that enable efficient DNA extraction and amplification with real-time monitoring capabilities, facilitating the efficient identification of corrosion-causing microbes. The present disclosure also relates to an RT-PCR based assay for the on-site detection and quantification of corrosion-causing microbes. The assay entails collecting samples from corroded surfaces or surrounding environments, followed by microbial DNA extraction utilizing a specific DNA isolation bio-buffer, PCR augmenter and PCR buffer composition. During the RT-PCR assay, specific primers targeting conserved genetic regions associated with microbial corrosion are employed for amplification. The assay of the present disclosure is simple and user-friendly making it well-suited for rapid and accurate detection of corrosion causing microbes in real field applications."

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