MUMBAI, India, April 17 -- Intellectual Property India has published a patent application (202631043601 A) filed by Brainware University, Kolkata, West Bengal, on April 6, for 'a rapid colorimetric diagnostic composition and method for detecting viable mycobacterium tuberculosis using enzyme-mediated activation of isoniazid.'
Inventor(s) include Mr. Agniz Dutta; and Dr. Sandhimita Mondal.
The application for the patent was published on April 17, under issue no. 16/2026.
According to the abstract released by the Intellectual Property India: "The present invention relates to a rapid colorimetric diagnostic composition and method for detecting viable Mycobacterium tuberculosis based on enzyme-mediated activation of isoniazid. The invention utilizes the KatG enzyme expressed by metabolically active bacterial cells to convert isoniazid into reactive intermediates, which subsequently induce a visible color change in a tetrazolium-based indicator. The system enables differentiation between live and non-viable bacteria by detecting metabolic activity rather than genetic material or structural components. The composition may be provided in a lyophilized form, ensuring stability and storage at ambient temperature. Upon addition of a biological sample such as sputum, the assay produces a detectable color change, preferably from yellow to purple, within approximately 30 minutes to 1 hour. The invention further allows preliminary assessment of isoniazid resistance through the use of a KatG inhibitor control, thereby offering a rapid, cost-effective, and field-deployable diagnostic solution."
Disclaimer: Curated by HT Syndication.
