MUMBAI, India, May 1 -- Intellectual Property India has published a patent application (202641048609 A) filed by Koneru Lakshmaiah Education Foundation, Hyderabad, Telangana, on April 16, for 'a method for simultaneous bioanalytical determination of semaglutide and liraglutide in human plasma using green lc-ms/ms technique with stable isotope-labelled internal standards and hybrid sample preparation.'
Inventor(s) include Anil Kollapareddy; Sreedhar Gundekari; Mohan Varkolu; Kousrali Sayyad; and Leela Prasad Kowtharapu.
The application for the patent was published on May 1, under issue no. 18/2026.
According to the abstract released by the Intellectual Property India: "The present invention relates to a green and highly sensitive bioanalytical method for the simultaneous determination of GLP-1 receptor agonists, namely semaglutide and liraglutide, in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method employs stable isotope-labelled internal standards to enhance accuracy and reproducibility while compensating for matrix effects and analytical variability. A hybrid sample preparation technique combining protein precipitation and solid-phase extraction is utilized to improve analyte recovery, reduce interference, and enhance sensitivity. The chromatographic separation is achieved using a gradient elution system with a volatile mobile phase comprising formic acid and acetonitrile, ensuring compatibility with mass spectrometric detection and adherence to green chemistry principles. The method demonstrates high specificity, low limits of quantification, and compliance with regulatory guidelines, making it suitable for pharmacokinetic studies, bioequivalence assessment, and therapeutic drug monitoring of GLP-1 analogs in clinical applications."
Disclaimer: Curated by HT Syndication.
