Intellectual Property India Publishes Patent Application for 'A Method For Isolation, Characterization And In-Silico Validation Of Cellulase-Producing Bacteria From Wastewater' Filed by Ashoka Institute Of Technology And Management; Mr. Abhishek Chaubey; Ms. Kaynat Salimi; Ms. Afia Aleeza Khan; Ms. Pranjal Yadav; Ms. Soumya Singh; Er. Ritesh Jaiswal; Er. Shubham Yadav; and Dr. Sarika Shrivastava

MUMBAI, India, March 13 -- Intellectual Property India has published a patent application (202611010228 A) filed by Ashoka Institute Of Technology And Management; Mr. Abhishek Chaubey; Ms. Kaynat Salimi; Ms. Afia Aleeza Khan; Ms. Pranjal Yadav; Ms. Soumya Singh; Er. Ritesh Jaiswal; Er. Shubham Yadav; and Dr. Sarika Shrivastava, Varanasi, Uttar Pradesh, on Jan. 31, for 'a method for isolation, characterization and in-silico validation of cellulase-producing bacteria from wastewater.'

Inventor(s) include Mr. Abhishek Chaubey; Ms. Kaynat Salimi; Ms. Afia Aleeza Khan; Ms. Pranjal Yadav; Ms. Soumya Singh; Er. Ritesh Jaiswal; Er. Shubham Yadav; and Dr. Sarika Shrivastava.

The application for the patent was published on March 13, under issue no. 11/2026.

According to the abstract released by the Intellectual Property India: "The present invention relates to a method for isolation, characterization, and in-silico validation of cellulase-producing bacteria from domestic and industrial wastewater sources. The method comprises collecting wastewater samples, isolating bacterial strains through serial dilution and culturing techniques, and screening the isolates for cellulolytic activity using carboxymethyl cellulose agar medium. Selected isolates are further evaluated based on qualitative cellulase activity and subjected to morphological and biochemical characterization. Molecular identification of potent isolates is performed through 16S rRNA gene amplification, sequencing, and phylogenetic analysis. The method further includes retrieval of cellulase amino acid sequences corresponding to the identified bacterial isolates and computational analysis of physicochemical properties, secondary structure prediction, and three-dimensional structural modeling. In-silico validation includes model quality assessment and molecular docking studies to analyze enzyme-substrate interactions with cellulose. The disclosed method enables systematic identification and validation of cellulase-producing bacteria with potential applicability in biomass degradation, bioenergy production, and industrial bioprocesses."

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