MUMBAI, India, May 29 -- Intellectual Property India has published a patent application (202641063484 A) filed by Vellore Institute Of Technology, Vellore, Tamil Nadu, on May 19, for 'a fermentation medium for producing nisin by lactococcus lactis and the method for preparing the same.'
Inventor(s) include Sahaya Shalmiya X; and Dr. Sreeja S.
The application for the patent was published on May 29, under issue no. 22/2026.
According to the abstract released by the Intellectual Property India: "The present invention relates to a fermentation medium for economical production of nisin using Lactococcus lactis. The medium substitutes costly components of a conventional de Man-Rogosa-Sharpe (MRS) medium with low-cost agro-industrial substrates while maintaining equivalent carbon and nitrogen availability. Molasses is utilized as a sole carbon source in place of glucose and is dosed based on total sugar determination to provide a concentration comparable to that of the conventional MRS medium. Fish protein hydrolysate is employed as a sole nitrogen source replacing yeast extract, peptone, and meat extract, and is added in quantities providing nitrogen availability equivalent to the conventional nitrogen sources. The remaining salts, buffers, and growth factors of the conventional medium are retained. The invention provides a method for producing nisin by preparing the modified medium, inoculating with Lactococcus lactis, fermenting under controlled static conditions, and recovering nisin from the culture supernatant. The developed medium enables efficient nisin production while significantly reducing fermentation cost."
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