MUMBAI, India, Sept. 28 -- Intellectual Property India has published a patent application (202611092828 A) filed by Deepti Agrawal on July 30, 2026, for “direct Estimation And Extraction Procedure For Total Indicator Of Microbial Biomass And Total Amino Profiling Via An Extended Short-Wavelength Chromogenic Activator Reagent Matrix (deepti Method)”.
Inventor includes Deepti Agrawal.
The application for the patent was published on September 25, 2026, under issue no. 39/2026.
Abstract: The present invention discloses a rapid, high-throughput, and eco-friendly colorimetric method (designated as the DEEPTI Method) for the direct estimation, verification, and molecular differentiation of total amino functional groups and microbial biomass in biological matrices. The process utilizes a multi-purpose dual biochemical probe operating via a Proprietary Chromogenic Activator Reagent (PCAR) matrix combined with customized fluid extraction vehicles (PEC-X and PEC- Y) under an optimized 1-hour processing protocol. Spectrophotometric analysis across an extended short-wavelength range from 400 nm to 600 nm yields distinct spectral signatures, displaying an immediate colorimetric shift to deep Ruhemann’s Purple for Primary Amines and a highly reproducible Light-Yellow Complex for Secondary Amines (including L-Proline and Hydroxyproline). Validated under a strict Randomized Block Design (RBD), the method eliminates hazardous traditional procedures, demonstrates a statistically significant expansion in raw extraction yield, and yields a 99.43% reduction in operational testing expenses compared to commercial kits.
Disclaimer: Curated by HT Syndication.
